Bioengineering of Bacterial Polymer Inclusions Catalyzing the Synthesis of N -Acetylneuraminic Acid
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چکیده
منابع مشابه
Bioengineering of bacterial polymer inclusions catalyzing the synthesis of N-acetylneuraminic acid.
N-Acetylneuraminic acid is produced by alkaline epimerization of N-acetylglucosamine to N-acetylmannosamine and then subsequent condensation with pyruvate catalyzed by free N-acetylneuraminic acid aldolase. The high-alkaline conditions of this process result in the degradation of reactants and products, while the purification of free enzymes to be used for the synthesis reaction is a costly pro...
متن کاملBioengineering of bacterial polymer inclusions 1 catalyzing the synthesis of N - acetyl neuraminic acid
David O. Hooks, Paul A. Blatchford, Bernd H. A. Rehm 3 4 Institute of Fundamental Sciences and MacDiarmid Institute of Advanced Materials 5 and Nanotechnology, Massey University, Private Bag 11222, Palmerston North, New 6 Zealand 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 # Corresponding author telephone: +64-6-350-5515, ext. 7890; fax: +64-6-350-2267; e-mail: [email protected]. Copyrigh...
متن کاملSynthesis of N-acetylneuraminic acid and of CMP-N-acetylneuraminic acid in the rat liver cell.
Adult male rats, under starving and normal conditions, were injected intravenously with N-acetyl[3H]mannosamine and after various time intervals the specific radioactivities of free N-acetylneuraminic acid (NeuAc) and CMP-N-acetylneuraminic acid were determined in the liver. The specific radioactivity of free NeuAc was high even within 20s after injection; the maximum was reached between 7 and ...
متن کاملColominic Acid, a Polymer of N-acetylneuraminic Acid
All acidic carbohydrate has been isolated from the culture medium of the enteric microorganism E. coli K(235) L(+)O. This substance has been named colominic acid. N-acetylneuraminic acid, which has been isolated from the hydrolysate of colominic acid, is believed to be the monomer unit from which colominic acid is constituted. Colominic acid is not antigenic in rabbits. Tests have failed to rev...
متن کاملCorrigendum: Characterization of a novel N-acetylneuraminic acid lyase favoring industrial N-acetylneuraminic acid synthesis process
The original version of this article contained an error in testing the kinetic parameters of CgNal towards pyruvate, in which the concentration of ManNAc (50 mM) was not in excess. This error was corrected by re-running the assay in the presence of excessive ManNAc (180 mM). The corrected kinetic parameters of CgNal towards pyruvate are shown in Table 1. These changes do not change the conclusi...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2013
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.03947-12